Sensitive electrochemical detection of NADH and ethanol at low potential based on pyrocatechol violet electrodeposited on single walled carbon nanotubes-modified pencil graphite electrode

In this work, the electrodeposition of pyrocatechol violet (PCV) was initially investigated by the electrochemical surface plasmon resonance (ESPR) technique. Subsequently, PCV was used as redox-mediator and was electrodeposited on the surface of pencil graphite electrode (PGE) modified with single-wall carbon nanotubes (SWCNTs). Owing to the remarkable synergistic effect of SWCNTs and PCV, PGE/SWCNTs/PCV exhibited excellent electrocatalytic activity towards dihydronicotinamide adenine dinucleotide (NADH) oxidation at low potential (0.2 V vs. SCE) with fast amperometric response (< 10 s), broad linear range (1.3-280 mu M), good sensitivity (146.2 mu A mM(-1) cm(-2)) and low detection limit (1.3 mu M) at signal-to-noise ratio of 3. Thus, this PGE/SWCNTs/PCV could be further used to fabricate a sensitive and economic ethanol biosensor using alcohol dehydrogenase (ADH) via a glutaraldehyde/BSA cross-linking procedure. (C) 2014 Elsevier B.V. All rights reserved.