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    Spectroscopic investigation of tetracycline interaction with phospholipid Langmuir-Blodgett films

    Year: 2002

    Journal: Materials Science and Engineering C 22 (2002) 301-305, 20111221

    Authors: G. Caminati, C. Focardi, G. Gabrielli, F. Gambinossi, B. Mecheri, M. Nocentini, M. Puggelli

    Organizations: a Dipartimento di Chimica, Universiti di Firenze, Polo Scientifico, Via della Lastruccia 3, 50019 Sesto Fiorentino, Firenze, Italy b Istituto Zooprofilattico, Sperimentale delle regioni Lazio e Toscana, Dipartimento di Firenze, Via di Castelpulci 41, 50010 San Martino alla Palma, Firenze, Italy

    A widely used veterinary antibiotic, tetracycline (TC), has been incorporated in Langmuir-Blodgett (LB) films of dipalmitoylphosphatidic acid (DPPA) by means of two different procedures: co-transfer and incubation in solution. The resulting structures were characterized by means of contact angle and ellipsometric measurements. The presence of the antibiotic in the phospholipid film was evidenced by means of UV-Vis electronic absorption and infrared vibrational spectroscopy. The two sets of measurements unambiguously indicated the presence of the drug in the LB layer films obtained with both methods, although incubation led to a smaller content of immobilized tetracycline. In both cases, the drug was found to reside in the hydrophilic portion of the layers due to specific interactions of the dimethylamino group of the molecule with the polar head groups of the phospholipid.