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    Surface modification using a novel type I hydrophobin HGFI

    Year: 2009

    Journal: Analytical and Bioanalytical Chemistry, Volume 394, Number 3 / June, 2009, 783-789, 20111221

    Authors: Sen Hou, Xinxin Li, Xiaoyu Li, Xi-Zeng Feng, Rui Wang, Chen Wang, Lei Yu and Ming-Qiang Qiao

    Organizations: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China, National Center for Nanoscience and Technology, Beijing, 100080, China, Institute of Molecular Biology, College of Life Science, Nankai University, Tianjin, 300071, China

    Surface wettability conversion with hydrophobins is important for its applications in biodevices. In this work, the application of a type I hydrophobin HGFI in surface wettability conversion on mica, glass, and poly(dimethylsiloxane) (PDMS) was investigated. X-ray photoelectron spectroscopy (XPS) and water-contact-angle (WCA) measurements indicated that HGFI modification could efficiently change the surface wettability. Data also showed that self-assembled HGFI had better stability than type II hydrophobin HFBI. Protein patterning and the following immunoassay illustrated that surface modification with HGFI should be a feasible strategy for biosensor device fabrication.