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    Surface Plasmon Resonance based sensing of lysozyme in serum on Micrococcus lysodeikticus-modified graphene oxide surfaces

    Year: 2017

    Journal: Biosens. Bioelectron., Volume 89, nov-15, page 525–531

    Authors: Vasilescu, Alina; Gaspar, Szilveszter; Gheorghiu, Mihaela; David, Sorin; Dinca, Valentina; Peteu, Serban; Wang, Qian; Li, Musen; Boukherroub, Rabah; Szunerits, Sabine

    Organizations: Marie Curie International Reintegration Grant [FP7 PEOPLE-2010-RG-277126]; Romanian Executive Unit for Higher Education, Research, Development and Innovation [PN II-ID-PCCE-2011-2-0075, PN II-RU-TE-2014-4-2434]; Centre National de la Recherche Scientifique (CNRS); University Lille 1; Nord Pas de Calais region; Institut Universitaire de France (IUF); Fundamental Research Funds of Shandong University [31370074614039]

    Keywords: Graphene oxide; Layer-by-layer; Whole cell biosensor; Lysozyme; Surface Plasmon Resonance; Serum

    Lysozyme is an enzyme found in biological fluids, which is upregulated in leukemia, renal diseases as well as in a number of inflammatory gastrointestinal diseases. We present here the development of a novel lysozyme sensing concept based on the use of Micrococcus lysodeikticus whole cells adsorbed on graphene oxide (GO)-coated Surface Plasmon Resonance (SPR) interfaces. M. lysodeikticus is a typical enzymatic substrate for lysozyme. Unlike previously reported sensors which are based on the detection of lysozyme through bioafflnity interactions, the bioactivity of lysozyme will be used here for sensing purposes. Upon exposure to lysozyme containing serum, the integrity of the bacterial cell wall is affected and the cells detach from the GO based interfaces, causing a characteristic decrease in the SPR signal. This allows sensing the presence of clinically relevant concentrations of lysozyme in undiluted serum samples. (C) 2016 Elsevier B.V. All rights reserved.
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