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    Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

    Year: 2008

    Journal: Colloids and Surfaces B: Biointerfaces, Volume 62, Issue 1, 15 March 2008, Pages 157-161, 20111221

    Authors: Jin-Won Parka, and Dong June Ahn

    Organizations: Gachon Bionano Research Institute, Kyungwon University, Bokjeongdong, Sengnamsi, Kyungkido 461-701, South Korea, Department of Chemical & Biological Engineering, Korea University, Anamdong 5 Ga, Seongbookgu, Seoul 136-701, South Korea

    Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir–Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.