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    The effect of poly (L-lactic acid) nanofiber orientation on osteogenic responses of human osteoblast-like MG63 cells

    Year: 2011

    Journal: Journal of the Mechanical Behavior of Biomedical Materials, Volume 4, Issue 4, May 2011, Pages 600-609, 20111221

    Authors: Bo Wang a,c , Qing Cai b , Shen Zhang b , Xiaoping Yang b,* , Xuliang Deng a,c

    Organizations: a Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, PR China, b Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China, c Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, PR China

    In this study, poly (L-lactic acid) (PLLA)/trifluoroethanol (TFE) solution was electrospun to fabricate fibrous scaffolds with different fiber orientations. Random and parallel PLLA nanofiber alignments were achieved by using a metal plate and a rolling rod as the receiver, respectively. The parallel PLLA fibrous scaffolds were further hot-stretched to obtain hyperparallel PLLA fibrous scaffolds. The PLLA fibrous scaffolds were characterized by fiber diameter, interfiber distance, fiber array angle, water contact angle, morphology and mechanical strength. The tensile strength of hyperparallel nano-fibers was approximately 5- and 14-times the parallel and random fibers, respectively. Osteoblast-like MG63 cells were cultured on the PLLA scaffolds to study the effects of fiber orientation on cell morphology, proliferation and differentiation. The cells on the randomly-oriented scaffolds showed irregular forms, while the cells exhibited shuttle-like shapes on the parallel scaffolds and had larger aspect ratios along the fiber direction of the hyperparallel scaffolds. Alkaline phosphatase (ALP) activity and collagen I (placeStateCol I) and osteocalcin (OC) deposition exhibited fiber orientation dependence. With an increase in parallelism of the fibers, there was a decrease in ALP activity and placeStateCol I and OC production. These results suggest that exploitation of PLLA fiber orientation may be used to control osteoblast-like cell responses.