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    The impact of dendrimer-grafted modifications to model silicon surfaces on protein adsorption and bacterial adhesion

    Year: 2011

    Journal: Biomaterials, Volume 32, Issue 35, December 2011, Pages 9168-9179, 20111207

    Authors: Eichler M. 1 2, Katzur V. 2, Scheideler L. 1, Haupt M. 3, Geis-Gerstorfer J. 1, Schmalz G 4, Ruhl S. 5, Müller R. 2, Rupp F. 1

    Last authors: Frank Rupp

    Organizations: 1 University Hospital Tuebingen, Department of Prosthetic Dentistry, Section Medical Materials and Technology, Osianderstr. 2-8, 72076 Tuebingen, Germany 2 Institute of Physical and Theoretical Chemistry, University of Regensburg, Universitaetsstr. 31, 93053 Regensburg, Germany 3 Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstr. 12, 70569 Stuttgart, Germany 4 Department of Operative Dentistry and Periodontology, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany 5 Department of Oral Biology, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY 14214-3092, USA

    Country: Germany, USA, US, United States, United States of America, America

    In the oral cavity, omnipresent salivary protein films (pellicle) mediate bacterial adhesion and biofilm formation on natural tissues as well as on artificial implant surfaces, which may cause serious infectious diseases like periimplantitis. The purpose of this in vitro study was to investigate the adsorption/desorption behaviour of human saliva on model surfaces grafted with polyamidoamine (PAMAM) dendrimer molecules compared to self-assembled monolayers (SAMs) exhibiting the same terminal functions (–NH2, –COOH) by two complementary analytical methods. Furthermore, the role of saliva conditioning of PAMAM and analogous SAM modifications on the adhesion of Streptococcus gordonii DL1, an early oral colonizer, was investigated. In contrast to SAMs, PAMAM-grafted surfaces showed reduced streptococcal adherence in the absence of pre-adsorbed saliva similar to the level obtained for poly(ethylene glycol) (PEG) coatings. Moreover, coatings of PAMAM-NH2 maintained their bacteria-repellent behaviour even after saliva-conditioning. As a general outcome, it was found that lower amounts of protein adsorbed on PAMAM coatings than on analogous SAMs. Since this study demonstrates that covalently bound PAMAM dendrimers can modulate the oral bacterial response, this approach has significant potential for the development of anti-adhesive biomaterial surfaces that are conditioned with proteinaceous films.