Contact
    Start Publications The influence of type-I collagen-coated PLLA aligned nanofibers ...
    ←  Publication Finder
    Attension

    The influence of type-I collagen-coated PLLA aligned nanofibers on growth of blood outgrowth endothelial cells

    Year: 2010

    Journal: Biomedical materials, Volume 5, Number 6, 20111221

    Authors: Zhang-Qi Feng 1 , Hui-Jun Lu 2 , Michelle K Leach 3 , Ning-Ping Huang 1 , Yi-Chun Wang 1 , Chang-Jian Liu 4 and Zhong-Ze Gu 1

    Organizations: 1 State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People’s Republic of China, 2 Department of Vascular Surgery, Wuxi People’s Hospital, Wuxi 214023, People’s Republic of China, 3 Department of Biomedical Engineering, The University of Michigan, Ann Arbor, MI 48109, USA, 4 Department of Vascular Surgery, The Af?liated Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, People’s Republic of China

    Nanofibrous scaffolds have been applied widely in tissue engineering to simulate the nanostructure of natural extracellular matrix (ECM) and promote cell bioactivity. The aim of this study was to design a biocompatible nanofibrous scaffold for blood outgrowth endothelial cells (BOECs) and investigate the interaction between the topography of the nanofibrous scaffold and cell growth. Poly(l-lactic acid) (PLLA) random and aligned nanofibers with a uniform diameter distribution were fabricated by electrospinning. NH3 plasma etching was used to create a hydrophilic surface on the nanofibers to improve type-I collagen adsorption; the conditions of the NH3 plasma etching were optimized by XPS and water contact angle analysis. Cell attachment, proliferation, viability, phenotype and morphology of BOECs cultured on type-I collagen-coated PLLA film (col-Film), random fibers (col-RFs) and aligned fibers (col-AFs) were detected over a 7 day culture period. The results showed that collagen-coated PLLA nanofibers improved cell attachment and proliferation; col-AFs induced the directional growth of cells along the aligned nanofibers and enhanced endothelialization. We suggest that col-AFs may be a potential implantable scaffold for vascular tissue engineering.