Contact
    Start Publications The interaction between OPH and paraoxon at the air-water ...
    ←  Publication Finder
    Attension

    The interaction between OPH and paraoxon at the air-water interface studied by AFM and epifluorescence microscopies

    Year: 2005

    Journal: Colloids and Surfaces B: Biointerfaces 40 (2005) 75-81, 20111221

    Authors: Xihui Cao, Mustapha Mabrouki , Sarita V. Mello , Roger M. Leblanc , Vipin K. Rastogi , Tu-Chen Cheng, Joseph J. DeFrank

    Organizations: a Department of Chemistry, University of Miami, Coral Gables, FL 33124, USA b US Army Edgewood Chemical & Biological Center, Aberdeen Proving Ground, MD 21010-5423, USA.

    The paraoxon hydrolysis reaction catalyzed by organophosphorus hydrolase (OPH) monolayer at the air-water interface was studied. OPH-paraoxon interactions, occurring at the two-dimensional interface, by close-packed, highly orientated OPH monolayer, were investigated by several different surface chemistry techniques; e.g. surface pressure area isotherms, atomic force microscopy (AFM), and in situ epifluorescence microscopy. The characterization of OPH Langmuir and Langmuir-Blodgett films prepared in both the presence and absence of paraoxon, demonstrated significantly distinctive feature when compared with one another. Continuous growth of the OPH aggregates is a distinct phenomenon associated with hydrolysis, in addition to the pH changes in the local environment of the enzyme macromolecules.