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    The osteogenic response of mesenchymal stem cells to an injectable PLGA bone regeneration system

    Year: 2013

    Journal: BiomaterialsVolume 34, Issue 37, December 2013, Pages 9352–9364, 20131119

    Authors: Judith M. Curran 1, Sandra Fawcett 1, Lloyd Hamilton 2, Nicholas P. Rhodes 1, Cheryl V. Rahman 2, Morgan Alexander 3, Kevin Shakesheff 2, John A. Hunt 1,

    Organizations: 1: Clinical Engineering, Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, UK2: Drug Delivery and Tissue Engineering, Centre for Biomolecular Sciences, University of Nottingham, UK3: Biophysics and Surface Analysis, Boots Science Building, University of Nottingham, UK

    The enrichment of substrates/surfaces with selected functional groups, methyl (–CH3), allyl amine (–NH2), allyl alcohol (–OH) and acrylic acid (–COOH), can be used to trigger mesenchymal stem (MSC) cell differentiation into specified lineages, minimising the need for exogenous biological supplementation. We present the successful translation of this research phenomenon to an injectable two phase injectable PLGA system, utilising plasma techniques, for the repair of bone defects. Modified microspheres were characterised using water contact angel (WCA), X-ray Photon Spectroscopy (XPS) and scanning electron microscopy (SEM). When cultured in contact with MSCs in vitro, the ability of the modified particles, within the 2 phase system, to induce differentiation was characterised using quantitative assays for cell viability and histological analysis for key markers of differentiation throughout the entirety of the three dimensional scaffold. Biological analysis proved that selected modified microspheres have the ability to induce MSC osteogenic (-NH2 modified scaffolds) and chondrogenic (–OH modified scaffolds) differentiation throughout the entirety of the formed scaffold. Therefore optimised plasma modification of microspheres is an effective tool for the production of injectable systems for the repair of bone and cartilage defects.