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    Tuning Cell Adhesion and Growth on Biomimetic Polyelectrolyte Multilayers by Variation of pH During Layer-by-Layer Assembly

    Year: 2013

    Journal: Macromolecular Bioscience Volume 13, Issue 10, pages 1327–1338, October 2013, 20140105

    Authors: Neha Aggarwal 1, Noomi Altgärde 2, Sofia Svedhem 2, Georgios Michanetzis 3, Yannis Missirlis 3, Thomas Groth 1,*

    Last authors: Thomas Groth

    Organizations: 1 Biomedical Materials Group, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany 2 Department of Applied Physics, Chalmers University of Technology, Göteborg, Sweden 3 Biomedical Engineering Laboratory, Faculty of Mechanical Engineering, University of Patras, Patras, Greece

    Country: Sverige, Sweden, Germany, Greece

    Polyelectrolyte multilayers of chitosan and heparin are assembled on glass where heparin is applied at pH = 4, 9 and 4 during the formation of the first layers followed by pH = 9 at the last steps (denoted pH 4 + 9). Measurements of wetting properties, layer mass, and topography show that multilayers formed at pH = 4 are thicker, contain more water and have a smoother surface compared to those prepared at pH = 9 while the pH = 4 + 9 multilayers expressed intermediate properties. pH = 9 multilayers are more cell adhesive and support growth of C2C12 cells better than pH = 4 ones. However, pH 4 + 9 conditions improve the bioactivity to a similar level of pH = 9 layers. Multilayers prepared using pH 4 + 9 conditions form thick enough layers that may allow efficient loading of bioactive molecules.