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    Ultrasensitive Detection of Human Liver Hepatocellular Carcinoma Cells Using a Label-Free Aptasensor

    Year: 2014

    Journal: Anal. Chem., 2014, 86 (10), pp 4956–4960, 20141008

    Authors: Leila Kashefi-Kheyrabadi †‡, Masoud A. Mehrgardi*†‡, Emilia Wiechec §, Anthony P. F. Turner †, and Ashutosh Tiwari †

    Last authors: Ashutosh Tiwari

    Organizations: † Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, Linköping University, Linköping S-58183, Sweden ‡ Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran § Department of Clinical and Experimental Medicine,Linköping University, Linköping S-58185, Sweden

    Country: Sweden, Sverige, Iran

    Liver cancer is one of the most common cancers in the world and has no effective cure, especially in later stages. The development of a tangible protocol for early diagnosis of this disease remains a major challenge. In the present manuscript, an aptamer-based, label-free electrochemical biosensor for the sensitive detection of HepG2, a hepatocellular carcinoma cell line, is described. The target cells are captured in a sandwich architecture using TLS11a aptamer covalently attached to a gold surface and a secondary TLS11a aptamer. The application of TLS11a aptamer as a recognition layer resulted in a sensor with high affinity for HepG2 cancer cells in comparison with control cancer cells of human prostate, breast, and colon tumors. The aptasensor delivered a wide linear dynamic range over 1 × 102 to 1 × 106cells/mL, with a detection limit of 2 cells/mL. This protocol provides a precise method for sensitive detection of liver cancer with significant advantages in terms of simplicity, low cost, and stability.