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    Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates

    Year: 2011

    Journal: Colloids and Surfaces B: Biointerfaces 2011, 87 (2) pp 409-414, 20121211

    Authors: Jarkko J. Heikkinen, Liisa Kivimäki, Juha A.E. Määttä, Inka Mäkelä, Leena Hakalahti, Kristiina Takkinen, Markku S. Kuloma, Vesa P. Hytönen, , Osmo E.O. Hormi

    Organizations: Department of Chemistry, University of Oulu, P.O. Box 3000, FI-90014 Oulu, Finland, VTT Technical Research Centre of Finland, P.O. Box 1100, FI-90571 Oulu, Finland, Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, FI-33014 Tampere, Finland, VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland

    A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimericavidin onto sol–gelsubstrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimericavidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimericavidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimericavidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.