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    Well-ordered thin films as practical components of biosensors

    Year: 2008

    Journal: Thin Solid Films, Volume 516, Issue 6, 30 January 2008, Pages 1171-1174, 20111221

    Authors: Joanna Cabaj, Krzysztof Idzik, Jadwiga Soloducho, Antoni Chyla, Jolanta Bryjak and Jacek Doskocz

    Organizations: Faculty of Medicinal Chemistry and Microbiology, Department of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland, Institute of Physical and Theoretical Chemistry, Department of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland, Faculty of Bioorganic Chemistry, Department of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland

    A Langmuir–Blodgett (LB) film consisting of glutaraldehyde as the binding site for enzyme molecule, an amphiphilic N-alkyl-bis(thiophene)carbazole (BTC7) as the additional electron mediator, and 22-tricosenoic acid (TA) as the matrix for these molecules were deposited on a hydrophilic substrate. Laccase was then covalently immobilized on the film via glutaraldehyde (GA). The sensitivity of this sensor was about twice as high as that of laccase sensor prepared with the same LB film as above but without the bis(thiophene)carbazole derivative. The laccase LB film exhibited enzyme activity.