Contact
    Start Publications A bifunctional bone scaffold combines osteogenesis and ...
    ←  Publication Finder
    Attension

    A bifunctional bone scaffold combines osteogenesis and antibacterial activity via in situ grown hydroxyapatite and silver nanoparticles

    Year: 2021

    Journal: Bio-Des. Manuf., Volume 4, SEP, page 452–468

    Authors: Yang, Youwen; Cheng, Yun; Deng, Fang; Shen, Lida; Zhao, Zhenyu; Peng, Shuping; Shuai, Cijun

    Organizations: National Natural Science Foundation of China [51935014, 82072084, 81871498]; Jiangxi Provincial Natural Science Foundation of China [20192ACB20005, 2020ACB214004]; Provincial Key R & D Projects of Jiangxi [20201BBE51012]; Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2018); Shenzhen Science and Technology Plan Project [JCYJ20170817112445033]; Innovation Team Project on University of Guangdong Province [2018GKCXTD001]; Technology Innovation Platform Project of Shenzhen Institute of Information Technology 2020 [PT2020E002]; Open Research Fund of Jiangsu Key Laboratory of Precision and Micro-Manufacturing Technology; China Postdoctoral Science Foundation [2020M682114]

    Keywords: Bone scaffold; In situ growth; Hydroxyapatite; Antibacterial properties; Surface modification

    Hydroxyapatite (HA) nanoparticles and silver (Ag) nanoparticles are expected to enable desirable bioactivity and antibacterial properties on biopolymer scaffolds. Nevertheless, interfacial adhesion between HA/Ag and the biopolymer is poor due to the large physicochemical differences between these components. In this study, poly L-lactic acid (PLLA) powder was first surface-modified with bioactive polydopamine (PDA) in an alkaline environment. Next, HA and Ag nanoparticles were grown in situ on the PDA-coated PLLA powder, which was then adhered to the porous bone scaffold using a selective laser-sintering process. Results showed that HA and Ag nanoparticles were homogenously distributed in the matrix, with enhanced mechanical properties. Simulated body fluid bioactivity tests showed that the in situ grown HA-endowed scaffold shows excellent bioactivity. In vitro tests confirmed that the scaffold exhibits favorable biocompatibility with human umbilical cord mesenchymal stem cells, as well as strong antibacterial activity against Gram-negative Escherichia coli. Furthermore, in vivo assays indicated that the scaffold promoted bone generation, with a new bone area fraction of 71.8% after 8 weeks' implantation, without inflammation.
    View publication