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    A liquid crystals-based sensing platform for detection of alpha-amylase coupled with destruction of host-guest interaction

    Year: 2019

    Journal: Colloid Surf. B-Biointerfaces, Volume 173, JAN 1, page 616–622

    Authors: Ma, Hui; Kang, Qi; Wang, Tao; Yu, Li

    Organizations: Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21373128]; Scientific and Technological Projects of Shandong Province of China [2018GSF121024]

    Keywords: Liquid crystals; alpha-Amylase; Sodium dodecyl sulphate; beta-Cyclodextrin; Host-guest; Interaction; Sensing platform

    A liquid crystals (LCs)-based sensing platform was constructed for simple, convenient, inexpensive and label-free detection of alpha-amylase, associated with disruption of host-guest interaction between sodium dodecyl sulphate (SDS) and beta-cyclodextrin (beta-CD). In the presence of SDS/beta-CD solution, a bright optical image was observed corresponding to the tilted anchoring of LCs at the aqueous/LCs interface. While a black optical appearance was captured when the pre-incubated mixture containing SDS/beta-CD complex and >= 0.0001 mg/mL alpha-amylase was transferred onto the fluid interface. The reason for this difference is that alpha-amylase could hydrolyze beta-CD and subsequently destroy the host-guest interaction between SDS and beta-CD. SDS molecules escaping from the cavity of beta-CDs were adsorbed at the aqueous/LCs interface, which evoked the homeotropic state of LCs. In the case of alpha-amylase below 0.0001 mg/mL, a bright optical image was observed. Based on these, detection of alpha-amylase could be achieved and its detection limit was similar to 0.0001 mg/mL (15 U/L). Moreover, this sensing platform was successfully utilized to monitor alpha-amylase in the body fluids, such as urine and saliva, indicating its potentiality in the relevant clinical diagnosis.
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