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    A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials

    Year: 2020

    Journal: J. Mat. Chem. B, Volume 8, FEB 14, page 1146–1156

    Authors: Rosqvist, E.; Niemela, E.; Frisk, J.; Oblom, H.; Koppolu, R.; Abdelkader, H.; Veliz, D. Soto; Mennillo, M.; Venu, A. P.; Ihalainen, P.; Aubert, M.; Sandler, N.; Wilen, C. -E.; Toivakka, M.; Eriksson, J. E.; Osterbacka, R.; Peltonen, J.

    Organizations: Abo Akademi University through the Centre of Excellence project - Functional Materials at Biological Interfaces; Tor, Joe and Pentti Borgs Minnesfond; Svenska Kulturfonden

    A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured Latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is Limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane Layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
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