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    Active Temperature Regulation and Teamed Boronate Affinity-Facilitated Microelectrode Module for Blood Glucose Detection in Physiological Environment

    Year: 2020

    Journal: Sens. Actuator B-Chem., Volume 324, DEC 1

    Authors: Xu, Xuechao; Liu, Chao; Zhang, Wen; Zou, Xiaobo

    Organizations: National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31972153]; National Key R&D Program of China [2017YFD0400102]; National Postdoctoral Funding of China [2018T110460, 2017M611739]; Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX18_2275]

    Keywords: Microelectrode module; Blood glucose detection; Physiological environment; Teamed boronate affinity; Active temperature regulation

    Physiological environment analysis is highly important to accurate detection of biological samples. In this work, a teamed boronate affinity-based molecular imprinting microelectrode module (MEM) with active temperature regulation was prepared for blood glucose detection in physiological environment. A silicon-based microelectrode array was utilized as working electrode, and arc-shaped electrodes on a printed circuit board contributed to a compact tri-electrode system. This module integrates a thermistor and a semiconductor cooler/heater, enabling active temperature regulation. Blood glucose were analyzed at physiological temperature. Physiological temperature retained primitiveness and avoided changing physicochemical properties of blood glucose. Meanwhile, constant temperature provided a constant adsorption capacity of functional materials towards glucose. Functional electrode materials, aminoferrocene-modified multi-walled carbon nanotubes (MWCNTs-AFC) and teamed boronate affinity-based molecular imprinting polymers modified multi-walled carbon nanotubes (MWCNTs-TBAMIPs), were filled in the measurement cell to form MWCNTs-TBA-MIPs/AFC MEM. MWCNTs-TBA-MIPs/AFC MEM detected glucose in physiological environment within 10 min and possessed a wide linear range (1 similar to 180 mu M) and a low LOD (0.61 mu M). With MWCNTs-TBA-MIPs/AFC MEM, blood glucose was determined selectively, eliminating the interferences from other co-existing molecules. Moreover, MWCNTs-TBA-MIPs/AFC MEM was employed to accurately measure the blood glucose in human serum, revealing its potential for biochemical detection.
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