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    Arginine-grafted porcine pericardium by copolymerization to improve the cytocompatibility, hemocompatibility and anti-calcification properties of bioprosthetic heart valve materials

    Year: 2022

    Journal: J. Mat. Chem. B, Volume 10, JUL 27, page 5571–5581

    Authors: Liang, Xuyue; Zheng, Cheng; Ding, Kailei; Huang, Xueyu; Zhang, Shumang; Lei, Yang; Yu, Kui; Wang, Yunbing

    Organizations: Chengdu Major Science and Technology Innovation Program [2019-YF08-00235-GX]; CAMS Innovation Fund for Medical Sciences [2021-I2M-5-013]; National Natural Science Foundation of China [32071357]; Sichuan Science and Technology Program [2021YFH0011]; National Key Research and Development Program, China [2020YFC1107802]; 111 Project of Introducing Talents of Discipline to Universities, China [B16033]

    Bioprosthetic heart valves (BHVs) have been used widely due to the development of transcatheter heart valve replacement technology. However, glutaraldehyde crosslinked pericardium (GA), which is widely used as a leaflet material for BHVs, still has disadvantages, including cytotoxicity, thrombosis, and calcification, which lead to the dysfunction and degeneration of BHVs. Herein, we prepared a methacrylated arginine-grafted BHV through the copolymerization of methacrylated arginine and methacrylated porcine pericardium (PP). Briefly, PP was crosslinked by glutaraldehyde and methacrylated polylysine (pLy-MA) to obtain methacrylated PP (pLy-GA), and the pLy-GA was then copolymerized with methacrylated arginine to prepare methacrylated arginine-grafted PP (pLy-GA-Arg). The introduction of Arg-MA improved the ability of PP to resist platelet adhesion, and compared with GA, platelet adhesion decreased by 78% which exhibited improved antithrombotic properties. pLy-GA-Arg exhibited improved cytocompatibility and the relative proliferation rate of HUVECs increased by 2 times compared with GA. After 60 days of subcutaneous implantation, the calcification degree of pLy-GA-Arg was significantly lower than that of GA (4.37 +/- 0.33 mu g mg(-1)versus 157.46 +/- 41.74 mu g mg(-1)). The introduction of arginine improved the hemocompatibility and cytocompatibility of PP and reduced its calcification, offering a potential option for BHV fabrication in the future.
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