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    Biosynthesis and characterization of a novel biocompatible medium chain length polyhydroxyalkanoate by Pseudomonas mendocina CH50 using coconut oil as the carbon source

    Year: 2018

    Journal: J. Mater. Sci.-Mater. Med., Volume 29, DEC

    Authors: Basnett, Pooja; Marcello, Elena; Lukasiewicz, Barbara; Panchal, Bijal; Nigmatullin, Rinat; Knowles, Jonathan C.; Roy, Ipsita

    Organizations: ReBioStent project - European Union's Seventh Programme for research, technological development and demonstration [604251]; HyMedPoly project - Horizon 2020 [643050]; Neurimp project [604450]

    Country: Athens, GREECE

    This study validated the utilization of triacylglycerides (TAGs) by Pseudomonas mendocina CH50, a wild type strain, resulting in the production of novel mcl-PHAs with unique physical properties. A PHA yield of 58% dcw was obtained using 20g/L of coconut oil. Chemical and structural characterisation confirmed that the mcl-PHA produced was a terpolymer comprising of three different repeating monomer units, 3-hydroxyoctanoate, 3-hydroxydecanoate and 3-hydroxydodecanoate or P(3HO-3HD-3HDD). Bearing in mind the potential of P(3HO-3HD-3HDD) in biomedical research, especially in neural tissue engineering, in vitro biocompatibility studies were carried out using NG108-15 (neuronal) cells. Cell viability data confirmed that P(3HO-3HD-3HDD) supported the attachment and proliferation of NG108-15 and was therefore confirmed to be biocompatible in nature and suitable for neural regeneration [GRAPHICS}
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