Contact
    Start Publications Bulk phase behaviour vs interface adsorption: Effects of anions ...
    ←  Publication Finder
    QSense

    Bulk phase behaviour vs interface adsorption: Effects of anions and isotopes on beta-lactoglobulin (BLG) interactions

    Year: 2021

    Journal: J. Colloid Interface Sci., Volume 598, SEP 15, page 430–443

    Authors: Fries, Madeleine R.; Skoda, Maximilian W. A.; Conzelmann, Nina F.; Jacobs, Robert M. J.; Maier, Ralph; Scheffczyk, Niels; Zhang, Fajun; Schreiber, Frank

    Organizations: Tubingen University/LISA+; Deutsche Forschungsgemeinschaft; ISIS Facility [RB1910571]

    Keywords: Anion; Isotope; Bulk; Adsorption; Wetting; QCM-D; NR

    Hypothesis: Protein adsorption is highly relevant in numerous applications ranging from food processing to medical implants. In this context, it is important to gain a deeper understanding of protein-protein and protein-surface interactions. Thus, the focus of this investigation is on the interplay of bulk properties and surface properties on protein adsorption. It was hypothesised that the type of solvent and ions in solution should significantly influence the protein's bulk and interface behaviour, which has been observed in literature and previous work for other net negatively charged, globular proteins such as bovine serum albumin (BSA). Experiments: The phase behaviour of beta-lactoglobulin (BLG) with lanthanum chloride (LaCl3) THORN and iodide (LaI3) normal water H2O(l) and heavy water (D2O(1) was established via optical microscopy and ultraviolet-visible spectroscopy. The formation of an adsorption layer and its properties such as thickness, density, structure, and hydration was investigated via neutron reflectivity, quartz-crystal microbalance with dissipation, and infra-red measurements. Findings: beta-lactoglobulin does not show significant anion-induced or isotope-induced effects - neither in bulk nor at the solid-liquid interface, which deviates strongly from the behaviour of bovine serum albumin. We also provide a comprehensive discussion and comparison of protein-specific bulk and interface behaviour between bovine serum albumin and beta-lactoglobulin dependent on anion, cation, solvent, and substrate properties. These findings pave the way for understanding the transition from adsorption to crystallisation. (C) 2021 Elsevier Inc. All rights reserved.
    View publication