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    Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection

    Year: 2018

    Journal: Appl. Biochem. Biotechnol., Volume 186, DEC, page 1061–1073

    Authors: Muenchen, Daniela Kunkel; Martinazzo, Janine; Brezolin, Alexandra Nava; de Cezaro, Alana Marie; Rigo, Aline Andressa; Mezarroba, Mateus Nava; Manzoli, Alexandra; Leite, Fabio de Lima; Steffens, Juliana; Steffens, Clarice

    Organizations: Cnpq; Capes; Fapergs; Finep

    Keywords: Zucchini; Enzymatic activity; Functionalization; Self-assembled monolayers (SAM); Nanobiosensor; Atomic force microscopy (AFM)

    A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10mgL(-1)) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028mgL(-1).
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