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    Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and beta-estradiol in Water

    Year: 2020

    Journal: Appl. Biochem. Biotechnol., Volume 190, APR, page 1512–1524

    Authors: de Cezaro, AM; Rigo, AA; Martinazzo, J; Muenchen, DK; Manzoli, A; Correa, DS; Steffens, J; Steffens, C

    Organizations: Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [00,1]; CnpqConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); FapergsFundacao de Amparo a Ciencia e Tecnologia do Estado do Rio Grande do Sul (FAPERGS); FinepFinanciadora de Inovacao e Pesquisa (Finep)

    Keywords: Water; Contaminants; Hormones; Enzyme; Sensitivity; Limit of detection

    This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17 beta-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/mu g, limit of detection of 0.1 and 0.4 ng/L for the hormones 17 beta-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices.
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