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    Cardiolipin-Containing Lipid Membranes Attract the Bacterial Cell Division Protein DivIVA

    Year: 2021

    Journal: Int. J. Mol. Sci., Volume 22, AUG

    Authors: Labajova, Nad'a; Baranova, Natalia; Jurasek, Miroslav; Vacha, Robert; Loose, Martin; Barak, Imrich

    Organizations: EMBO [8193]; VEGA from the Slovak Academy of Sciences [2/0001/21]; Slovak Research and Development Agency [APVV-18-0104]; Czech Science Foundation [GA20-20152S]; ERC [679239]; Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility (BIF); Life Science Facility (LSF); CEITEC 2020 [LQ1601]; Ministry of Education, Youths and Sports of the Czech Republic; National Programme for Sustainability II funds; programme Projects of Large Research, Development, and Innovations Infrastructures [LM2015042, LM2015085]; MEYS CR from the Large Infrastructures for Research, Experimental Development and Innovations [LM2015070]

    Keywords: Clostridioides difficile; DivIVA; lipid membrane; cardiolipin; phosphatidylglycerol

    DivIVA is a protein initially identified as a spatial regulator of cell division in the model organism Bacillus subtilis, but its homologues are present in many other Gram-positive bacteria, including Clostridia species. Besides its role as topological regulator of the Min system during bacterial cell division, DivIVA is involved in chromosome segregation during sporulation, genetic competence, and cell wall synthesis. DivIVA localizes to regions of high membrane curvature, such as the cell poles and cell division site, where it recruits distinct binding partners. Previously, it was suggested that negative curvature sensing is the main mechanism by which DivIVA binds to these specific regions. Here, we show that Clostridioides difficile DivIVA binds preferably to membranes containing negatively charged phospholipids, especially cardiolipin. Strikingly, we observed that upon binding, DivIVA modifies the lipid distribution and induces changes to lipid bilayers containing cardiolipin. Our observations indicate that DivIVA might play a more complex and so far unknown active role during the formation of the cell division septal membrane.
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