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    Characterization of monolayers and liposomes that mimic lipid composition of HeLa cells

    Year: 2020

    Journal: Colloid Surf. B-Biointerfaces, Volume 196, DEC

    Authors: Botet-Carreras, Adria; Teresa Montero, M.; Sot, Jesus; Domenech, Oscar; Borrell, Jordi H.

    Organizations: IN2UB [ART2017]; Spanish Ministry of Economy and Competitiveness [TEC2016-79156-P]; Catalan authorities (Generalitat de Catalunya) [214SGR 1442]

    Keywords: HeLa cells; Lipid monolayers; Fluorescence; Langmuir-Blodgett films; BAM; AFM

    In this work, based on several studies, we develop an artificial lipid membrane to mimic the HeLa cell membrane using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) and cholesterol (CHOL). This is then a means to further study the fusion process of specific engineered liposomes. To characterize the mimicked HeLa cell membrane, we determined a series of surface pressure-area (pi p-A) isotherms and the isothermal compression modulus was calculated together with the dipole moment normal to the plane of the monolayer. The existence of laterally segregated domains was assessed using a fluorescence technique (Laurdan) and two microscopy techniques: Brewster angle microscopy (BAM) and atomic force microscopy (AFM) of Langmuir-Blodgett films (LBs) extracted at 30 mN m(-1). To examine the nature and composition of the observed domains, force spectroscopy (FS) based on AFM was applied to the LBs. Finally, two engineered liposome formulations were tested in a fusion assay against mimicked HeLa cell membrane LBs, showing good results and thereby opening the door to further assays and uses.
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