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    Complement proteins regulating macrophage polarisation on biomaterials

    Year: 2019

    Journal: Colloid Surf. B-Biointerfaces, Volume 181, 1-Sep, page 125–133

    Authors: Araujo-Gomes, N.; Romero-Gavilan, F.; Zhang, Y.; Martinez-Ramos, C.; Elortza, F.; Azkargorta, M.; Martin de Llano, J. J.; Gurruchaga, M.; Goni, I.; van den Beucken, J. J. J. P.; Suay, J.

    Organizations: MINECO [MAT2017-86043-R]; Universitat Jaume I [Predoc/2014/25, UJI-B2017-37]; Basque GovernmentBasque Government [IT611-13, Predoc/2016/1/0141]; University of the Basque CountryUniversity of Basque Country [UFI11/56]; Basque Department of Industry, Tourism and Trade (Etortek program), ProteoRed-ISCIII [PRB3 IPT17/0019]; Basque Department of Industry, Tourism and Trade (Elkartek program), ProteoRed-ISCIII [PRB3 IPT17/0019]; CIBERehd Network [SEV-2016-0644]; Severo Ochoa Grant [SEV-2016-0644]

    Keywords: Complement system; Immune response; Proteomics; Dental implants; Hybrid sol-gel; Macrophage plasticity

    One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-alpha and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization.
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