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    Controlled Adhesion of HUVEC on Polyelectrolyte Multilayers by Regulation of Coating Conditions

    Year: 2021

    Journal: ACS Appl. Bio Mater., Volume 4, FEB 15, page 1441–1449

    Authors: Rudt, Alexander; Sun, Jing; Qin, Meng; Liu, Luo; Syldatk, Christoph; Barbeck, Mike; Xiong, Xin; Krastev, Rumen

    Organizations: program of Baden-Wurttemberg government: Innovative Projekte/Kooperationsprojekte an den Hochschulen Baden-Wurttemberg [2014.001]; German Federal Ministry of Education and Research (BMBF) [FKZ 3FH155PX6, FKZ 13GW0400C]; Europaische Fonds fur regionale Entwicklung EFRE [FKZ: 712879]; Ministerium fur Wirtschaft, Arbeit und Wohnungsbau Baden-Wurttemberg [FKZ: 712879]

    Keywords: polyelectrolyte multilayers; PSS; PAH; electrolyte concentration; internal excess charge; cell adhesion; cell viabili; protein adsorption

    Adhesion of host cells on the surface of implants is necessary for a healthy ingrowth of the implanted material. One possibility of surface modification is the coating of the implant with a second material with advantageous physical-chemical surface properties for the biological system. The coverage with blood proteins takes place immediately after implantation. It is followed by host-cell interaction on the surface. In this work, the effect of polyelectrolyte multilayer coatings (PEMs) on adhesion and activity of human umbilical vein endothelial cells (HUVECs) was studied. The PEMs were formed from poly(styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) from solutions Controlled Cell Adhesion with different concentrations of NaCl varying between 0 and 1.0 M. The adhesion of HUVEC and their viability on the PEM is related to the amount of adsorbed proteins from the applied cell growth medium. The amount of adsorbed proteins is controlled not only by the surface charge but also by the internal excess charge of the PEM. The internal excess charge of the PEM was controlled by changing the electrolyte concentration in the deposition solutions.
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