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    Controlled co-immobilization of biomolecules on quinone-bearing plasma polymer films for multifunctional biomaterial surfaces

    Year: 2020

    Journal: Plasma Process. Polym., Volume 17, NOV

    Authors: Czuba, Urszula; Moreno-Couranjou, Maryline; Collard, Delphine; De Pauw-Gillet, Marie-Claire; Quintana, Robert; Lassaux, Patricia; Detrembleur, Christophe; Choquet, Patrick

    Keywords: antibacterial coatings; biomaterials; dielectric barrier discharges; immobilization of biomolecules; plasma polymerization

    In this study, an efficient methodology, allowing the controlled co-immobilization of two complementary biomolecules, is reported for the production of multifunctional antibacterial surfaces. To promote long-lasting covalent immobilization, metallic surfaces are first coated with a quinone-bearing poly(methacrylate)-based thin film by combining an atmospheric pressure liquid-assisted plasma polymerization and a controlled sodium periodate-induced catechol oxidation steps. The influence of the oxidation step on the film morphology and chemistry is investigated using an analytical multitool approach involving atomic force microscopy, ultraviolet, infrared, and X-ray photoelectron spectroscopy techniques. Quartz crystal microbalance with dissipation monitoring (QCM-D) analyses allow the rapid determination of the optimal biomolecule immobilization conditions in terms of kinetics of grafting and biomolecule solution concentrations. In vitro functional assays combined with QCM-D analyses demonstrate promising, dual biologically active coated surfaces.
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