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    Controlled Drug Release and Cytotoxicity Studies of Beta-Lapachone and Doxorubicin Loaded into Cyclodextrins Attached to a Polyethyleneimine Matrix

    Year: 2020

    Journal: Int. J. Mol. Sci., Volume 21, AUG

    Authors: Kowalczyk, Agata; Kasprzak, Artur; Poplawska, Magdalena; Ruzycka, Monika; Grudzinski, Ireneusz P.; Nowicka, Anna M.

    Keywords: controlled drug release; cyclodextrin; lapachone; doxorubicin; polyethylenimine; cytotoxicity studies

    This work presents a new look at the application of cyclodextrins (CD) as a drug nanocarrier. Two different cyclodextrins (alpha CD,beta CD) were covalently conjugated to branched polyethylenimine (PEI), which was additionally functionalized with folic acid (PEI-beta CD-alpha CD-FA). Here, we demonstrated that the combination of alpha CD and beta CD enabled to load and control release of two anticancer drugs: doxorubicin (DOX) and beta-lapachone (beta-LP) (DOX in beta-CD and beta-LP into alpha-CD) via host-guest inclusion. The PEI-beta CD(DOX)-alpha CD-FA nanoconjugate was used to transport anticancer drugs into A549 lung cancer cells for estimation the cytotoxic and antitumor effect of this nanoconjugate. The presence of FA molecules should facilitate the penetration of studied nanoconjugate into the cell. Whereas, the non-cellular experiments proved that the drugs are released from the carrier mainly in the pH 4.0. The release mechanism is found to be anomalous in all studied cases.
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