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    Core-sheath nanostructured chitosan-based nonwovens as a potential drug delivery system for periodontitis treatment

    Year: 2020

    Journal: Int. J. Biol. Macromol., Volume 142, JAN 1, page 521–534

    Authors: dos Santos, Danilo M.; Chagas, Paulo A. M.; Leite, Ilaiali S.; Inada, Natalia M.; de Annunzio, Sarah R.; Fontana, Carla R.; Campana-Filho, Sergio P.; Correa, Daniel S.

    Organizations: Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) from BrazilFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2017/20973-4, 2017/12174-4, 2013/07276-1, 2018/23015-7, 2018/09088-1]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) from BrazilConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); MCTI-SisNano from Brazil [CNPq/402.287/2013-4]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES) from BrazilCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]; Rede Agronano (EMBRAPA) from Brazil

    Keywords: Chitosan; Coaxial electrospinning; Nanocarrier

    Core-sheath nanofibers were successfully prepared via coaxial electrospinning by using chitosan with well-defined structural characteristics as the shell layer and poly (vinyl alcohol) (PVA) containing tetracycline hydrochloride (TH) as the core layer. The effects of the average degree of deacetylation ((DD) over bar) of chitosan and the post-electrospinning genipin crosslinking on physicochemical and biological properties of resulting nonwovens were evaluated. Defect-free and geometrically uniform nanofibers with diameters predominantly in the range of 100-300 nm were prepared, and transmission electron microscopy (TEM) revealed the core-sheath structures and its preservation after crosslinking. The mechanical properties, as well as the stability of nonwovens in aqueous medium, were greatly improved by genipin-crosslinking, which enabled a sustained release of TH over 14 days. Results also revealed that the release profile of TH in the presence of lysozyme was affected by the composition of the shell layer, as the TH release rate increases with decreasing of (DD) over bar. Further in vitro antimicrobial activity demonstrated that the cross-linked nonwovens containing TH showed strong activity against bacterial strains associated with periodontal disease. Additionally, the nonwovens did not demonstrate cytotoxic toward fibroblast (HDFn) cells, hence showing their potential for applications as a novel drug delivery platform for periodontitis treatment. (C) 2019 Elsevier B.V. All rights reserved.
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