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    Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol

    Year: 2019

    Journal: Int. J. Biol. Macromol., Volume 122, FEB 1, page 1144–1151

    Authors: Pereira, Joao R.; Araujo, Diana; Marques, Ana C.; Neves, Luisa A.; Grandfils, Christian; Sevrin, Chantal; Alves, Vitor D.; Fortunato, Elvira; Reis, Maria A. M.; Freitas, Filomena

    Organizations: Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO); Associated Laboratory for Sustainable Chemistry-Clean Processes and Technologies - LAQV; Centro de Investigacao de Materiais I i3N; LEAF-Linking Landscape, Environment, Agriculture and Food - FCT/MCTES [UID/Multi/04378/2013, UlD/QUI/50006/2013, UID/CTM/50025/2013, PEst-OE/AGR/U10245/2014]; ERDF, under the FT2020 Partnership Agreement [POCI-01-0145-FEDER-007728, POCI-01-0145-FEDER-007265, POCI-01-0145-FEDER-007688]; FCT [IF/00505/2014, IF/00589/2015]; FCT-MCTES [BD/115173/2016]

    Keywords: Polyhydroxyalkanoates (PHA); Pseudomonas chlororaphis; Adhesion

    Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 +/- 1.8 mol%), 3-hydroxydecanoate (29 +/- 3.1 mol%), 3-hydroxytetradecanoate (12 +/- 0.4 mol%), 3-hydroxyoctanoate (10 +/- 1.5 mol%) and 3-hydroxyhexanoate (6 +/- 0.3 mol%). The biopolymer had an average molecular weight of 1.1 x 10(5) Da, with a polydispersity index of 15, and was semi-crystalline, as shown by its crystallinity index of 37 +/- 0.2%. It had low melting (44 degrees C) and glass transition (-48 degrees C) temperatures, and was thermally stable up to 285 degrees C. The biopolymer films were elastic and translucid, were hydrophobic and presented relatively high permeability to oxygen and carbon dioxide. The films demonstrated to have good adhesion properties towards porcine skin and human skin. The tension (61.1 +/- 20.6 kPa) and shear (12.7 +/- 2.14 kPa) bond strength of the mcl-PHA for porcine skin suggest its potential as a biomaterial for the development of novel natural adhesives for wound closure or wound dressings. (C) 2018 Elsevier B.V. All rights reserved.
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