Contact
    Start Publications Design of a Bio-Based Device for Micro Total Analysis Combining ...
    ←  Publication Finder
    Attension

    Design of a Bio-Based Device for Micro Total Analysis Combining Fused Deposition Modeling and Layer-by-Layer Technologies

    Year: 2020

    Journal: Macromol. Mater. Eng., Volume 305, NOV

    Authors: de Leon, Alberto S.; de Frutos, Raquel M.; Molina, Sergio I.

    Organizations: Ministry of Science, Innovation and Universities [TEC2017-86102-C2-2-R, FJCI-2016-29330]; Junta de Andalucia (Research group INNANOMAT)Junta de Andalucia [TEP-946]; CEIMAR [CEIJ-007]; EUEuropean Commission

    Keywords: bio-based polymers; fused deposition modeling; layer-by-layer technologies; micro total analysis; surface modification

    In this paper a methodology is presented to fabricate a micropatterned device made of bio-based polymers (polylactic acid, sodium alginate (ALG), and chitosan (CHI)) suitable for micro total analysis. Fused deposition modeling and layer-by-layer technologies are combined to create tailorable devices with submillimetric controlled well size that can be rapidly prepared in house. As proof of concept, the influence of immobilization of ALG/CHI bilayers in the selective adsorption of alkaline phosphatase and its activity is studied. Due to the noncovalent nature of these interactions, it is proven that these devices can be reused for at least three times, contributing to generate less waste. It is foreseen that the combination of these fast and inexpensive technologies will lead to new setups for laboratory and clinical assays such as high-throughput screening where controlled immobilization of enzymes, peptides, or other valuable biomolecules needs to be used.
    View publication