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    Development of Cantilever Nanoimmunosensors Applied to the Detection of beta-Estradiol and Estrone in Water

    Year: 2020

    Journal: IEEE Sens. J., Volume 20, NOV 1, page 12620–12627

    Authors: Cezaro, AM; Rigo, AA; Martinazzo, J; Muenchen, DK; Brezolin, AN; Manzoli, A; Correa, DS; Steffens, J; Steffens, C

    Organizations: National Council for Scientific and Technological Development (CNPq), BrazilConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); Coordination for the Improvement of Higher Education Personnel (CAPES), BrazilCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]; Research Support Foundation of the State of Rio Grande of Sul (FAPERGS), Brazil; Financiadora de Estudos e Projetos (Finep)Financiadora de Inovacao e Pesquisa (Finep); URI; EmbrapaEmpresa Brasileira de Pesquisa Agropecuaria (EMBRAPA)

    Keywords: Water; contaminants; hormones; functionalization

    Endocrine-disrupting are chemical substances that have hormonal activity and can potentially cause diseases to the endocrine system in animals, including humans. Therefore, there is a necessity of to develop sensitive, specific, and reliable analytical systems capable to detect compounds using low-cost and portable devices. In this study, were developed cantilever nanoimmunosensors for beta-estradiol and estrone hormones detection in tap water. The cantilever nanoimmunosensorswere functionalizedwith anti-beta-estradiol and anti-estrone antibodies. The device surface was analyzed by scanning electron microscopy, atomic force microscopy, and polarization modulation infrared reflection absorption spectroscopy and showed an efficient functionalization with conformational changes. Results demonstrated a linear relationship in a range between 0.01 and 50 mu g/L. A limit of detection of 9 and 26 ng/L (0.009 and 0.026 ppb) and sensitivity of 0.390 and 0.140 V/ng were observed for beta-estradiol and estrone hormones using the cantilever nanoimmunosensors functionalized anti-beta-estradiol and antiestrone, respectively. These results demonstrate that these devices are suitable to be apply in tap water for quality monitoring and detection beta-estradiol and estrone.
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