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    Dual effect of non-ionic detergent Triton X-100 on insulin amyloid formation

    Year: 2019

    Journal: Colloid Surf. B-Biointerfaces, Volume 173, JAN 1, page 709–718

    Authors: Siposova, Katarina; Sedlak, Erik; Kozar, Tibor; Nemergut, Michal; Musatov, Andrey

    Organizations: Slovak Grant Agency VEGAVedecka grantova agentura MSVVaS SR a SAV (VEGA) [2/0009/17]; Slovak Research and Development AgencySlovak Research and Development Agency [APVV-15-453, APVV-15-0069, APVV SK-TW 17-0012]; Structural Fund of EU [26220120033, 26210120002]; MVTS [SAS-MOST JRP2017-6]

    Keywords: Insulin; Amyloid aggregation; Morphology of fibrils; Triton X-100-insulin interaction

    Atomic force microscopy, Thioflavin T (ThT) fluorescence assay, circular dichroism spectroscopy, differential scanning calorimetry, and molecular modeling techniques have been employed to investigate the amyloid aggregation of insulin in the presence of non-ionic detergent, Triton X-100 (TX-100). In contrast to recently described Inhibition of lysozyme amyloid formation by non-ionic detergents (Siposova, 2017), the amyloid aggregation of insulin in the presence of sub-micellar TX-100 concentration exhibits two dissimilar phases. The first, inhibition phase, is observed at the protein to detergent molar ratio of 1:0.1 to 1:1. During this phase, the insulin amyloid fibril formation is inhibited by TX-100 up to similar to 60%. The second, "morphological" phase, is observed at increasing detergent concentration, corresponding to protein:detergent molar ratio of similar to 1:1 - 1:10. Under these conditions a significant increase of the steady-state ThT fluorescence intensities and a dramatically changed morphology of the insulin fibrils were observed. Increasing of the detergent concentration above the CMC led to complete inhibition of amyloidogenesis. Analysis of the experimental and molecular modeling results suggests an existence of up to six TX-100 binding sites within dimer of insulin with different binding energy. The physiological relevance of the results is discussed.
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