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    Dynamical and noninvasive monitoring of curcumin effect on the changes in the viscoelasticity of human breast cancer cells: A novel model to assess cell apoptosis

    Journal: Talanta, Volume 236, JAN 1

    Authors: Zhou, Bin; Hao, Yan; Wang, Zhiyong; Wei, Pei; Du, Lianfeng; Xia, Qiang

    Organizations: National Natural Science Foundation of China [82160352]; Science and Technology Plan Project of Guizhou Province, China [qiankehepingtairencai [2018] 5772-019, qiankehejichu-ZK [2021] yiban 484]; Science and Technology Fund Project of Guizhou Provincial Health Commission, China [gzwjkj2020-1-039]; Major Research Project of Innovation Group in Education Department of Guizhou Province, China [KY [2016] 037]

    Keywords: Quartz crystal microbalance cytosensor; Dynamical and noninvasive monitoring; Energy dissipation; Cell viscoelasticity; Apoptosis degree

    A real-time quartz crystal microbalance (QCM) cytosensor was first developed for dynamical and noninvasive monitoring of cell viscoelasticity for evaluation of apoptosis degree. In this work, human breast cancer cells MCF-7 and MDA-MB-231 were employed as cell model and respectively captured on the surface of QCM electrode modified with mercaptosuccinic acid and poly-L-lysine. Cell viscoelasticity was measured dynamically by real-time monitoring energy dissipation with QCM, and the dynamic diagram of the energy dissipation of MDA-MB-231 cells treated with curcumin was first obtained. The results displayed that the changes of energy dissipation in MDA-MB-231 cells and MCF-7 cells were 8.81 x 10(-6) and 5.29 x 10(-6), particularly due to the difference in cell viscoelasticity. Furthermore, curcumin was used to induce cell apoptosis and suppress energy dissipation of MDA-MB-231 cells. Combining apoptosis assay with QCM measurement, the results revealed good linear relationship between cell viscoelasticity inhibition and apoptosis rate with correlation coefficient R = 0.9908. The QCM cytosensor could rapidly, accurately, dynamically, and noninvasively monitor the changes of cell viscoelasticity for evaluation of apoptosis degree in MDA-MB-231 cells. The study established a new model for cell apoptosis assessment, facilitating understanding of the mechanisms of cell apoptosis on the aspect of mechanical properties.
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