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    Effect of selected B-ring-substitute doxysterols on artificial model erythrocyte membrane and isolated red blood cells

    Year: 2020

    Journal: Biochim. Biophys. Acta-Biomembr., Volume 1862, FEB 1

    Authors: Targosz-Korecka, M; Writrzak, A; Chachaj-Brekiesz, A; Gonet-Surowka, A; Kubisiak, A; Filiczkowska, A; Szymonski, M; Dynarowicz-Latka, P

    Keywords: Langmuir monolayers; RBC model membrane; Oxysterols; AFM; Nanoindentation

    In this paper, systematic studies concerning the influence of selected oxysterols on the structure and fluidity of human erythrocyte membrane modeled as Langmuir monolayers have been performed. Three oxidized cholesterol derivatives, namely 7 alpha-hydroxycholesterol (7 alpha-OH) 7 beta-hydroxycholesterol (7 beta-OH) and 7-ketocholesterol (7-K) have been incorporated in two different proportions (10 and 50%) into artificial erythrocyte membrane, modeled as two-component (cholesterol:POPC) Langmuir monolayer. All the studied oxysterols were found to alter membrane fluidity and the effect was more pronounced for higher oxysterol content. 7 alpha-OH increased membrane fluidity while opposite effect was observed for 7 beta-OH and 7-K. Experiments performed on model systems have been verified in biological studies on red blood cells (RBC). Consistent results have been found, i.e. under the influence of 7 alpha-OH, the elasticity of erythrocytes increased, and in the presence of other investigated oxysterols - decreased. The strongest effect was noticed for 7-K. Change of membrane elasticity was associated with the change of erythrocytes shape, being most noticeable under the influence of 7-K.
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