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    Enzyme activity of thiophene-fluorene based-copolymer blended with urease in thin films

    Year: 2020

    Journal: Colloid Surf. A-Physicochem. Eng. Asp., Volume 603, OCT 20

    Authors: Rodrigues, RD; de Aquino, TP; Caseli, L; Peres, LO

    Organizations: FAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2018/18180-9, 2018/04340-4, 2014/50869-6]; CNPqConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [401109/2014-3, 303358/2014-9]; CAPES (Education Ministry) via the projects of the National Institute for Science and Technology on Organic Electronics (INEO) [23038.000776/201754]; CAPESCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]

    Keywords: Conjugated polymer; Urease; Biosensor; Spin coating

    Enzyme activity can be boosted if the molecular architecture of films is manipulated. In this paper, we show an alternative way to immobilize the enzyme urease in a matrix composed of the copolymer poly [(9,9-dioctylfluorene)-co-thiophene] by using the spin coating method. The films were characterized with Fluorescence, UV-vis, and Infrared spectroscopies, confirming not only the adsorption of the enzyme, but also the maintenance of its secondary structure. Enzyme activity of the blended films was evaluated in terms of hydrolysis of urea as a heterogeneous catalyst. As measured with UV-vis spectroscopy, the blended films presented substrate-enzyme affinity in relation to the Langmuir-Blodgett method, which may be important for the production of enzymatic sensors with enhanced performance. This increase was related to the configurational disorder in the copolymer/enzyme blend with their chains being intertwined, facilitating the access of urea to the catalytic site of the enzyme.
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