Contact
    Start Publications EPS for bacterial anti-adhesive properties investigated on a ...
    ←  Publication Finder
    QSense

    EPS for bacterial anti-adhesive properties investigated on a model metal surface

    Year: 2022

    Journal: Colloid Surf. B-Biointerfaces, Volume 213, MAY

    Authors: Zanna, Sandrine; Mercier, Dimitri; Gardin, Elise; Allion-Maurer, Audrey; Marcus, Philippe

    Keywords: EPS; Pseudoalteromonas; Metal surface; X-ray photoelectron spectroscopy (XPS); Quartz crystal microbalance (QCM)

    Understanding Extracellular Polymeric Substances (EPS) interaction on a well-defined chromium surface is of importance especially for biocorrosion processes. Adsorption of EPS extracted from Pseudoalteromonas NCIMB 2021 on Cr surfaces was investigated using in situ quartz crystal microbalance (QCM) and X-ray photoelectron spectroscopy (XPS). We show that EPS adsorption is an irreversible process. The amount of adsorbed EPS in-creases with increasing EPS concentration in solution. For low EPS concentration, the surface is only partially covered by EPS, whereas a continuous organic film of around 15 nm is formed at the surface for high EPS concentrations. An in-depth structuration of this organic layer is evidenced with a strong enrichment of proteins in the inner part and of polysaccharides in the outer part. Adhesion of Pseudoalteromonas NCIMB 2021 has been tested on Cr surfaces covered or not by EPS extracted from Pseudoalteromonas NCIMB 2021. EPS conditioning with a 15 nm film inhibits bacterial adhesion on Cr, showing that this organic film has efficient anti-adhesive properties.
    View publication