The aim of the present study is to determine the long term effects of the Cold Atmospheric Plasma (CAP) treatment on the Grade 5 Titanium(Ti) implant surface at different storage conditions. After CAP application, Ti discs were stored in room conditions, in saline and an inert ambient, then contact angle and surface roughness measurements were done. Discs were stored using accelerated aging test for 1 month, 3 months, 6 months, 1 year and 2 years and measurements were made at the end of these time points. Optical Emission Spectra (OES) measurement was performed to determine plasma generate species during CAP application. OES shows that OH and NO generated. As a result of contact angle measurements, showed that the samples stored in saline were capacity of preserving hydrophilicity for an extended period of time compared to the samples stored in room condition, inert ambient. Surface roughness measurements with a profilometer showed no difference in surface roughness compared to the control group (untreated Ti) in plasma-treated groups.