Contact
    Start Publications Evaluation of Meibum Lipid Composition According to Tear ...
    ←  Publication Finder
    KSV NIMA

    Evaluation of Meibum Lipid Composition According to Tear Interferometric Patterns

    Year: 2022

    Journal: Am. J. Ophthalmol., Volume 240, AUG, page 37–50

    Authors: Jun, Ikhyun; Kim, Sehui; Kim, Hyoseon; Kim, Sun Woong; Ji, Yong Woo; Kim, Kwang Pyo; Lee, Tai Gyu; Seo, Kyoung Yul

    Organizations: Basic Science Research Program [NRF-2021R1I1A1A01047951, NRF-2019R1F1A1063311]; Korea Mouse Phenotyping Project [NRF-2013M3A9D5072551]; National Research Foundation - Ministry of Science, ICT, and Future Planning

    circle PURPOSE: To determine the relationship between tear film interferometric patterns and properties of lipid, including rheological properties. circle DESIGN: Prospective, cross-sectional laboratory inves-tigation. circle METHOD: This study included 105 subjects (94 dry eye patients and 11 normal participants). The subjects were divided into 3 categories (group 1, normal; group 2, thin; and group 3, irregular) according to interferometric pat-terns. According to tear interferometric patterns, ultra-performance liquid chromatography (LC) quadrupole-linear ion trap/mass spectrometry (MS)-based analysis was used to investigate lipid profiling of meibum. Rhe-ological properties were examined by using a LangmuirBlodgett trough with saline solution. circle RESULTS: Normal subjects showed Pearl-like patterns, and dry eye patients showed either irregular or thin pat-terns. Group 2 tended to be the evaporative type, and group 3 tended to be the aqueous-deficient type. Lipid profiling using LC-MS identified 280 lipid species of 25 lipid classes. In the meibum of the patient groups, the con-tent of cholesteryl esters and nonpolar lipids was lower than that in the normal group. However, the content of polar lipids such as sphingolipids and phospholipids in the patient groups was higher than that in the normal group. Rheological properties showed that the lift-off areas were comparable among the 3 groups and the surface tension was the highest in group 1, followed by group 3 and group 2. circle CONCLUSIONS: The findings of this study suggest that tear interferometric patterns are associated with lipid pro-filing of meibum and its rheological properties. These results may contribute toward the development of new treatment modalities. (Am J Ophthalmol 2022;240: 37-50. (c) 2022 Elsevier Inc. All rights reserved.)
    View publication