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    Heavy metals detection in river water with cantilever nanobiosensor

    Year: 2020

    Journal: J. Environ. Sci. Health Part B-Pestic. Contam. Agric. Wastes, Volume 55, MAR 3, page 239–249

    Authors: Rigo, AA; De Cezaro, AM; Muenchen, DK; Martinazzo, J; Manzoli, A; Steffens, J; Steffens, C

    Organizations: National Council for Scientific and Technological Development (Cnpq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]; Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (Fapergs)Fundacao de Amparo a Ciencia e Tecnologia do Estado do Rio Grande do Sul (FAPERGS); Financiadora de Estudos e Projetos (Finep)Financiadora de Inovacao e Pesquisa (Finep); EmbrapaEmpresa Brasileira de Pesquisa Agropecuaria (EMBRAPA); URI

    Keywords: Self-assembled monolayers (SAM); surface; detection limit; phosphatase alkaline; water

    Heavy metals can be highly toxic depending on the dose and the chemical form. In this context, sensing devices such as nanobiosensors have been presented as a promising tool to monitor contaminants at micro and nanoscale. In this work, cantilever nanobiosensors with phosphatase alkaline were developed and applied to detect heavy metals (Pb, Ni, Cd, Zn, Co, and Al) in river water. The nanobiosensor surface was functionalized by the self-assembled monolayers (SAM) technique using 16-mercaptohexadecanoic acid, N-(3-dimethylaminopropyl)-N '-ethylcarbodiimide (EDC) and N- hydroxysuccinimide (NHS), and phosphatase alkaline enzyme. The sensing layer deposited on the cantilever surface presented a uniform morphology, at nanoscale, with 80 nm of thickness. The nanobiosensor showed a detection limit in the ppb range and high sensitivity, with a stability of fifteen days. The developed cantilever nanobiosensor is a simple tool, suitable for the direct detection of contaminants in river water.
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