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    Highly Sensitive Protein Detection by Asymmetric Mach-Zehnder Interferometry for Biosensing Applications

    Year: 2020

    Journal: ACS Appl. Bio Mater., Volume 3, JUL 20, page 4566–4572

    Authors: Goodwin, Melissa J.; Besselink, Geert A. J.; Falke, Floris; Everhardt, Arnoud S.; Cornelissen, Jeroen J. L. M.; Huskens, Jurriaan

    Keywords: biosensing; asymmetric Mach-Zehnder interferometry; quartz crystal microbalance; surface plasmon resonance; poly-l-lysine

    The sensitivity and performance of an asymmetric Mach-Zehnder interferometer (aMZI) were compared to those of quartz crystal microbalance with dissipation (QCM-D). The binding of streptavidin to sensor chips coated with poly-L-lysine (PLL), modified with biotin and oligoethyleneglycol (OEG) (PLLbiotin), was used to compare the binding signals obtained from both technologies. PLL-biotin proved to be an efficient method to add bioreceptors to both the QCM-D and aMZI chips. The final, saturated value of streptavidin binding was compared with those from aMZI (253 ng cm(-2)) and QCM-D (460 ng cm(-2)). These values were then used to evaluate that 45% of the measured streptavidin mass in the QCM-D came from hydrodynamically coupled water. Importantly, the signal-to-noise ratio of the aMZI was found to be 200 times higher than that of the QCM-D. These results indicate the potential of the aMZI platform for highly sensitive and accurate biosensing applications.
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