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    How the replacement of cholesterol by 25-hydroxycholesterol affects the interactions with sphingolipids: The Langmuir Monolayer Study complemented with theoretical calculations

    Year: 2021

    Journal: J. R. Soc. Interface, Volume 18, MAR 17

    Authors: Kobierski, Jan; Wnetrzak, Anita; Chachaj-Brekiesz, Anna; Filiczkowska, Anna; Petelska, Aneta D.; Dynarowicz-Latka, Patrycja

    Organizations: PL-Grid Infrastructure; Polish Ministry of Science and Higher Education subvention [2020-N17/MNS/000019]; European Funds for Regional Development; National Funds of Ministry of Science and Higher Education, as part of the Operational Program Development of Eastern Poland 2007-2013 [POPW.01.03.00-20044/11]

    Keywords: Langmuir monolayers; interactions; sphingolipids; oxysterols

    In this paper, a representative of chain-oxidized sterols, 25-hydroxycholesterol (25-OH), has been studied in Langmuir monolayers mixed with the sphingolipids sphingomyelin (SM) and ganglioside (GM(1)) to build lipid rafts. A classical Langmuir monolayer approach based on thermodynamic analysis of interactions was complemented with microscopic visualization of films (Brewster angle microscopy), surface-sensitive spectroscopy (polarization modulation-infrared reflection-absorption spectroscopy) and theoretical calculations (density functional theory modelling and molecular dynamics simulations). Strong interactions between 25-OH and both investigated sphingolipids enabled the formation of surface complexes. As known from previous studies, 25-OH in pure monolayers can be anchored to the water surface with a hydroxyl group at either C(3) or C(25). In this study, we investigated how the presence of additional strong interactions with sphingolipids modifies the surface arrangement of 25-OH. Results have shown that, in the 25-OH/GM(1) system, there are no preferences regarding the orientation of the 25-OH molecule in surface complexes and two types of complexes are formed. On the other hand, SM enforces one specific orientation of 25-OH: being anchored with the C(3)-OH group to the water. The strength of interactions between the studied sphingolipids and 25-OH versus cholesterol is similar, which indicates that cholesterol may well be replaced by oxysterol in the lipid raft system. In this way, the composition of lipid rafts can be modified, changing their rheological properties and, as a consequence, influencing their proper functioning.
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