Contact
    Start Publications Hybrid bilayer membranes on metallurgical polished aluminum
    ←  Publication Finder
    Attension

    Hybrid bilayer membranes on metallurgical polished aluminum

    Year: 2021

    Journal: Sci Rep, Volume 11, MAY 6

    Authors: Sabirovas, Tomas; Valiuniene, Ausra; Valincius, Gintaras

    Organizations: European Regional Development Fund under Promotion of Centers of Excellence and Innovation and Technology Transfer Centers program [01.2.2-CPVA-K-703, 01.2.2-CPVA-K-703-03-0010]

    In this work we describe the functionalization of metallurgically polished aluminum surfaces yielding biomimetic electrodes suitable for probing protein/phospholipid interactions. The functionalization involves two simple steps: silanization of the aluminum and subsequent fusion of multilamellar vesicles which leads to the formation of a hybrid bilayer lipid membrane (hBLM). The vesicle fusion was followed in real-time by fast Fourier transform electrochemical impedance spectroscopy (FFT EIS). The impedance-derived complex capacitance of the hBLMs was approximately 0.61 mu F cm(-2), a value typical for intact phospholipid bilayers. We found that the hBLMs can be readily disrupted if exposed to>400 nM solutions of the pore-forming peptide melittin. However, the presence of cholesterol at 40% (mol) in hBLMs exhibited an inhibitory effect on the membrane-damaging capacity of the peptide. The melittin-membrane interaction was concentration dependent decreasing with concentration. The hBLMs on Al surface can be regenerated multiple times, retaining their dielectric and functional properties essentially intact.
    View publication