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    Hydrophobin-Coated Solid Fluorinated Nanoparticles for F-19-MRI

    Journal: Adv. Mater. Interfaces, Volume 9, JUN

    Authors: Ayaz, Nazeeha; Dichiarante, Valentina; Pigliacelli, Claudia; Repossi, Jacopo; Gazzera, Lara; Borreggio, Marta; Maiolo, Daniele; Chirizzi, Cristina; Bergamaschi, Greta; Chaabane, Linda; Fasoli, Elisa; Metrangolo, Pierangelo; Bombelli, Francesca Baldelli

    Organizations: Regione Lombardia POR FESR [1175999]; MIUR [2017MYBTXC]; Politecnico di Milano within the CRUI-CARE Agreement; Italian Ministry of Health [GR-2016-02361325]

    Keywords: F-19-MRI; coatings; fluorinated nanoparticles; fluorine; hydrophobin; protein corona; self-assembly

    In recent years, fluorine-magnetic resonance imaging (F-19-MRI) has emerged as a promising diagnostic technique, complementary to traditional proton magnetic resonance imaging (H-1-MRI) and easily translatable for clinical use, providing in-depth in vivo quantification without the use of radioactive agents. This creates a need for the development of appropriate delivery systems for highly omniphobic fluorinated probes. The use of the film-forming protein hydrophobin (HFBII) represents a sustainable and simple method to invert the philicity of fluorinated surfaces. Here, the ability of HFBII to form a rigid protein monolayer on superfluorinated coatings rendering them hydrophilic is shown, a property that is also retained in biological environment. This approach is then translated to directly disperse a solid superfluorinated F-19-MRI probe, PERFECTA, in aqueous solution through the formation of core-shell hydrophobin stabilized PERFECTA nanoparticles (NPs). The obtained NPs are fully characterized in terms of morphology, magnetic properties, colloidal stability, protein corona formation, cellular viability, and imaging performance.
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