Contact
    Start Publications Imprinted hydrogels with LbL coating for dual drug release from ...
    ←  Publication Finder
    QSense

    Imprinted hydrogels with LbL coating for dual drug release from soft contact lenses materials

    Year: 2021

    Journal: Mater. Sci. Eng. C-Mater. Biol. Appl., Volume 120, JAN

    Authors: Silva, Diana; de Sousa, Herminio C.; Gil, Maria Helena; Santos, Luis F.; Amaral, Renata A.; Saraiva, Jorge A.; Salema-Oom, Madalena; Alvarez-Lorenzo, Carmen; Serro, Ana Paula; Saramago, Benilde

    Organizations: COMPETE 2020, FEDER; Fundacao para a Ciencia e a Tecnologia (FCT) [UID/QUI/00100/2019, UIDB/00100/2020, PTDC/CTM-BIO/3640/2014, UID/QUI/00062/2019, UID/QUI/50006/2020]; FEDER, within the PT2020 Partnership Agreement; Fundacao para a Ciencia e a Tecnologia [PD/BD/114088/2015]; MINECO (Spain) [SAF2017-83118-R]; Agencia Estatal de Investigacion (AEI, Spain); Xunta de Galicia (Spain) [AEMAT ED431E 2018/08]; FEDER

    Keywords: Layer-by-layer; Molecular imprinting; Controlled dual drug release; Moxifloxacin; Diclofenac; Soft contact lenses

    A combined strategy to control the release of two drugs, one anti-inflammatory (diclofenac sodium, DCF) and one antibiotic (moxifloxacin hydrochloride, MXF), from a soft contact lens (SCL) material, was assessed. The material was a silicone-based hydrogel, which was modified by molecular imprinting with MXF and coated by the layer-by-layer (LbL) method using natural polyelectrolytes: alginate (ALG), poly-L-lysine (PLL) and hyaluronate (HA), crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). Imprinting was used to increase the amount of MXF loaded and to sustain its release, while the LbL coating acted as a diffusion barrier for DCF and improved the surface properties. The drugs were loaded by soaking in a DCF + MXF dual solution. High hydrostatic pressure (HHP) was successfully applied in the sterilization of the drug-loaded hydrogels. The transmittance, refractive index, wettability and ionic permeability of the hydrogels remained within the required levels for SCLs application. The concentrations of the released DCF and MXF stayed above the IC50 and the MIC (for S. aureus and S. epidermidis) values, for 9 and 10 days, respectively. No ocular irritancy was detected by the HET-CAM test. NIH/3T3 cell viability demonstrated that the drug-loaded hydrogels were not toxic, and cell adhesion was reduced.
    View publication