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    Influence of amphotericin B on the thermodynamic properties and surface morphology of saturated phospholipid monolayer with different polar head at the air-water interface

    Year: 2021

    Journal: Colloid Surf. A-Physicochem. Eng. Asp., Volume 617, MAY 20

    Authors: Wang, Juan; Zhu, Hao

    Organizations: Scientific research project of Shaanxi Education Department [20JK0961]; Special Doctoral Fund of Xijing University [XJ17T02]

    Keywords: Amphotericin B; The electrically neutral phospholipid; The net negatively charged phospholipid; Monolayer

    Amphotericin B is not only a widely used antifungal drug, but also a potential novel immunotherapy anticancer drug. But it has great side effects on human body due to its pore-forming activity on cell?s membrane. Some researchers have found that AmB can form pores on the phospholipid monolayer in the absence of sterol. However, the effect of different polar heads of phospholipids on the pore-forming activity of AmB on the membrane is rarely reported. In this work, the influence of amphotericin B on the thermodynamic properties and surface morphology of saturated phospholipid monolayer with different polar head at the air-water interface have been studied. The results suggested that the intermolecular force between AmB and DPPC or DPPE molecules was attractive but the force between AmB and DPPG or DPPS molecules was repulsive. No matter what kind of phospholipid?s polar head, AmB can enhance the disorder of the AmB/phospholipid mixed monolayer. But at 30 mN/m, this effect of AmB from strong to weak was in the presence of PS head, PG head, PC head and PE head. And the drug can induce the phase state of the phospholipid monolayer to change from LC phase to LC-LE phase at 30 mN/m. Differently, the shape and distribution of LC phase domains in the mixed monolayer film with non-charged head group were different from that with negatively charged head group, which may be due to the intermolecular force between AmB and different phospholipid. The results are helpful to understand the influence of AmB on the domains without sterols on cell membrane, to lay the foundation for the interaction between
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