Contact
    Start Publications Influence of levofloxacin and clarithromycin on the structure ...
    ←  Publication Finder
    KSV NIMA

    Influence of levofloxacin and clarithromycin on the structure of DPPC monolayers

    Year: 2019

    Journal: Biochim. Biophys. Acta-Biomembr., Volume 1861, 1-Oct

    Authors: Ortiz-Collazos, Stephanie; Picciani, Paulo H. S.; Oliveira Jr, Osvaldo N.; Pimentel, Andre S.; Edler, Karen J.

    Organizations: FAPERJCarlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ) [210.558/2015, E-26/010.001241/2016]; National Council for Scientific and Technological Development (CNPq)National Council for Scientific and Technological Development (CNPq) [465259/2014-6, 302554/2017-3]; Sao Paulo Research Foundation (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [210.558/2015, 2014/50983-3, 2013/14262-7]; CAPESCAPES [88881.132891/2016-01]

    Keywords: Lung surfactant model; Monolayer; Macrolide; Fluoroquinolone; Phospholipids; Neutron reflectometry

    Research on lipid/drug interactions at the nanoscale underpins the emergence of synergistic mechanisms for topical drug administration. The structural understanding of bio-mimetic systems employing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as a lung surfactant model mixed with antibiotics, as well as their biophysical properties, is of critical importance to modulate the effectiveness of therapeutic agents released directly to the airways. In this paper, we investigate the structural details of the interaction between Levofloxacin, 'a respiratory quinolone', and the macrolide Clarithromycin, with DPPC monolayers at the air-water interface, using a combination of Brewster angle microscopy, polarization modulation-infrared reflection-adsorption spectroscopy (PM-IRRAS), surface pressure isotherms and neutron reflectometry (NR) to describe the structural details of this interaction. The results allowed association of changes in the pi-A isotherm profile with changes in the molecular organization and the co-localization of the antibiotics within the lipid monolayer by NR measurements. Overall, both antibiotics are able to increase the thickness of the acyl tails in DPPC monolayers with a corresponding reduction in tail tilt as well as to interact with the phospholipid headgroups as shown by PM-IRRAS experiments. The effects on the DPPC monolayers are correlated with the physical-chemical properties of each antibiotic and dependent on its concentration.
    View publication