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    Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

    Year: 2019

    Journal: Arch. Biochem. Biophys., Volume 663, 1-Mar, page 192–198

    Authors: Andrade, Marco A. R.; Derradi, Rafael; Simao, Ana M. S.; Millan, Jose Luis; Ramos, Ana P.; Ciancaglini, Pietro; Bolean, Mayte

    Organizations: Fundacan de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/20946-3, 2014/11941-3, 2015/00345-3, 2016/21236-0, 2017/08892-9]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - Brasil (CAPES)CAPES [001]; Conselho Nacional de Desenvolvimento Cientifico e TecnologicoNational Council for Scientific and Technological Development (CNPq)

    Keywords: Alkaline phosphatase; Cell membrane models; Langmuir monolayers; Langmuir-Blodgett films; Biomineralization

    Tissue-nonspecific alkaline phosphatase (TNAP) is a key enzyme in the biomineralization process as it produces phosphate from a number of phospho-substrates stimulating mineralization while it also inactivates inorganic pyrophosphate, a potent mineralization inhibitor. We have previously reported on the reconstitution of TNAP on Langmuir monolayers as well as proteoliposomes. In the present study, thin films composed of dimyristoylphosphatidic acid (DMPA) were deposited on titanium supports by the Langmuir-Blodgett (LB) technique, and we determined preservation of TNAP's phosphohydrolytic activity after incorporation into the LB films. Increased mineralization was observed after exposing the supports containing the DMPA:TNAP LB films to solutions of phospho-substrates, thus evidencing the role of TNAP on the growth of calcium phosphates after immobilization. These coatings deposited on metallic supports can be potentially applied as osteoconductive materials, aiming at the optimization of bone-substitutes integration in vivo.
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