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    Lipopolysaccharide O-antigen molecular and supramolecular modifications of plant root microbiota are pivotal for host recognition

    Year: 2022

    Journal: Carbohydr. Polym., Volume 277, FEB 1

    Authors: Vanacore, Adele; Vitiello, Giuseppe; Wanke, Alan; Cavasso, Domenico; Clifton, Luke A.; Mahdi, Lisa; Asuncion Campanero-Rhodes, Maria; Solis, Dolores; Wuhrer, Manfred; Nicolardi, Simone; Molinaro, Antonio; Marchetti, Roberta; Zuccaro, Alga; Paduano, Luigi; Silipo, Alba

    Organizations: PRIN 2017 Glytunes (2019-2022) [2017XZ2ZBK]; European Research Council (ERC) under the European Union [851356]; Spanish Ministry of Science, Innovation, and Universities [RTI2018-099985-B-I00]; CIBER of Respiratory Diseases (CIBERES) , an initiative from the Spanish Institute of Health Carlos III (ISCIII); Cluster of Excellence on Plant Sciences (CEPLAS) - Deutsche For-schungsgemeinschaft (DFG, German Research Foundation) [390686111, EXC 2048/1]; SPP DECRyPT [ZU 263/11-1]

    Keywords: Lipopolysaccharide; Plant microbiota; Herbaspirillum; Structure-function relationship; NMR

    Lipopolysaccharides, the major outer membrane components of Gram-negative bacteria, are crucial actors of the host-microbial dialogue. They can contribute to the establishment of either symbiosis or bacterial virulence, depending on the bacterial lifestyle. Plant microbiota shows great complexity, promotes plant health and growth and assures protection from pathogens. How plants perceive LPS from plant-associated bacteria and discriminate between beneficial and pathogenic microbes is an open and urgent question. Here, we report on the structure, conformation, membrane properties and immune recognition of LPS isolated from the Arabidopsis thaliana root microbiota member Herbaspirillum sp. Root189. The LPS consists of an O-methylated and variously acetylated Drhamnose containing polysaccharide with a rather hydrophobic surface. Plant immunology studies in A. thaliana demonstrate that the native acetylated O-antigen shields the LPS from immune recognition whereas the O- deacylated one does not. These findings highlight the role of Herbaspirillum LPS within plant-microbial crosstalk, and how O-antigen modifications influence membrane properties and modulate LPS host recognition.
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